*Target (protein/gene name): Wall Teichoic Acid beta-glycosyltransferase (SAV0258) *NCBI Gene # or RefSeq#: *Protein ID (NP or XP #) or Wolbachia#: *Organism (includingstrain): Staphylococcus aureus Etiologic Risk Group (see link below): (link not working?) Disease Information Link to TDR Targets page (if present): n/a Link to Gene Database page (NCBI): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156392/
Essentiality of this protein: Teichoic Acid beta-glycosyltransferase aids in Staphylococcus aureus resistance to Methicillin. Is it a monomer or multimer as biological unit? (make prediction athttp://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): Complex of proteins?: Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
Enzyme Assay company -- -or link (or citation) to paper that contains assay information -- links to assay reagents (substrates) pages. --- List cost and quantity of substrate reagents, supplier, and catalog #
Structure (PDB or Homology model) -- PDB # or closest PDB entry if using homology model: 5TZB -- For Homology Model option: 5TZ8 5TZE 5TZI 5TZJ 5TZK 5U02
Current Inhibitors: UDB-N-Acetylglucosamine Expression Information (has it been expressed in bacterial cells): Purification Method Based on the Histidine tag at the end, I believe the current purification method is similar to that of which we used in the Protein Purification Lab; using Imidazol and Ni-NTA. Image of protein (PyMol with features delineated and shown separately): *Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGIDKLVPRGSAAAALEHHHHHH
*length of your protein in Amino Acids: 368 Molecular Weight 42261.84 Extinction coefficient 33810 TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it:
TargetSp17- Wall Teichoic Acid beta-glycosyltransferase tarS (Staphylococcus aureus)
*Target (protein/gene name): Wall Teichoic Acid beta-glycosyltransferase (SAV0258)
*NCBI Gene # or RefSeq#:
*Protein ID (NP or XP #) or Wolbachia#:
*Organism (including strain): Staphylococcus aureus
Etiologic Risk Group (see link below): (link not working?)
Disease Information
Link to TDR Targets page (if present): n/a
Link to Gene Database page (NCBI):
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5156392/
Essentiality of this protein: Teichoic Acid beta-glycosyltransferase aids in Staphylococcus aureus resistance to Methicillin.
Is it a monomer or multimer as biological unit? (make prediction at http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html):
Complex of proteins?:
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
*EC#: 2.4.1
Link to BRENDA EC# page:
http://www.brenda-enzymes.org/enzyme.php?ecno=2.4.1.70
Enzyme Assay
company
-- -or link (or citation) to paper that contains assay information
-- links to assay reagents (substrates) pages.
--- List cost and quantity of substrate reagents, supplier, and catalog #
Structure (PDB or Homology model)
-- PDB # or closest PDB entry if using homology model: 5TZB
-- For Homology Model option: 5TZ8 5TZE 5TZI 5TZJ 5TZK 5U02
Current Inhibitors: UDB-N-Acetylglucosamine
Expression Information (has it been expressed in bacterial cells):
Purification Method Based on the Histidine tag at the end, I believe the current purification method is similar to that of which we used in the Protein Purification Lab; using Imidazol and Ni-NTA.
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCSTDQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETLKDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRTALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVSPEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEEQPQWINALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGKLNIQFNEGEPYFEGIDKLVPRGSAAAALEHHHHHH
*length of your protein in Amino Acids: 368
Molecular Weight 42261.84
Extinction coefficient 33810
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it:
Gene Sequence
*GC% Content for gene:
output
*GC% Content for gene (codon optimized):